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KMID : 0624620090420040206
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BMB Reports
2009 Volume.42 No. 4 p.206 ~ p.211
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Molecular cloning, tissue distribution and quantitative analysis of two proopiomelanocortin mRNAs in Japanese flounder (Paralichthys olivaceus)
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Kim Kyoung-Sun
Kim Hyun-Woo
Chen Thomas T.
Kim Young-Tae
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Abstract
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Proopiomelanocortin (POMC) plays an essential role in the stress response of the hypothalamic-pituitary-adrenal axis, and is the precursor of biologically active peptides such as adrenocorticotropin (ACTH), α-melanocyte-stimulating hormone (α-MSH), β-melanocyte-stimulation hormone (β-MSH) and β-endorphin. We have synthesized two different forms of POMC cDNA clones, POMC-I and POMC-II, from a pituitary cDNA library for Paralichthys olivaceus, or Japanese flounder. jfPOMC-I cDNA consists of 954bp and encodes a polypeptide of 216 amino acid residues, whereas jfPOMC-II consists of 971bp which encode a polypeptide of 194 amino acid residues. The high levels of jfPOMC-I and -II mRNAs detected in the pituitary tissue and moderate levels detected in the brain tissue plus our quantitative RT-PCR analysis, which showed there to be no significant difference between the levels of jfPOMC-I and -II mRNAs, indicate that there may be no functional separation between these two mRNAs in the flounder.
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KEYWORD
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Flounder, Molecular cloning, Paralichthys olivaceus, Pituitary, Proopiomelanocortin
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